I will have to go back to my validation binder to get the specifics for you.....plus am a little hesitant in that I do not want to make a definitive negative statement concerning Grifols testing (as I myself have not come to a negative conclusion). For the most part, it is working as expected. But I can answer some of the other issues you raised. So when the Erytra is questioning whether or not a Screen is Positive (calls it an NRD), then you have to make a decision. You can look at the card (or picture....but as stated previously, I tend to shy away from those giant pictures); and perhaps you decide to change the NRD to Negative....or you can do further work. At least at this moment, we choose to do further work based on both our validation results and on our admitted need to become more used to how we interpret the Grifols Cards , but also just the fact that it is new and I want to kind of continue correlation studies a little longer. So we would not interpret that questionable reaction just based on looking at the card. We would perform a panel (Grifols; or Immucor) and/or PeG testing.
As far as other screens we have pursued further....if we run a patient on the Erytra who has known antibodies (and was reactive when last tested) and it is Negative, we will either perform a PeG Screen, or run some Immucor cells in Ortho IgG (which is better as far as comparison studies than using PeG). So I guess we are still just "learning" about the Erytra (and I am feeling better about it the more we use it...AND, the more I do these comparison studies). Another thing to keep in mind is that when we did our initial comparison studies, a lot of those were frozen known antibody specimens, so perhaps those just didn't run as well on the Erytra as other techniques....because as I stated, it is getting better.
Hope that helps a little....sorry for not being more specific on antibodies but don't know how much could be due to being new users and/or using older, frozen specimens....so would not want to make too many associations with the Erytra at this point in time. But will keep you guys posted should I become more concerned....just wanted to throw it out initially so you would all also do due diligence when doing your comparison studies.
Thanks
Brenda