Malcolm Needs ☆ Posted May 30, 2018 Share Posted May 30, 2018 Well, the first thing I would say is that you would be wasting time and reagents to cross-match at the hospital, for the precise reason you quote; of course the units will be incompatible, so just perform an "immediate spin" cross-match (if you must) or perform an electronic issue, as you say that you trust RCI results. As far as antibodies directed against low prevalence antigens are concerned (the antibodies are usually quite common, it is the antigen that is usually quite rare), I would advise you to read Garratty G. How concerned should we be about missing antibodies to low incidence antigens? Transfusion 2003; 43 (7): 844-847, which, basically says what I said above - i.e. that NO TRANSFUSION IS 100% SAFE, but such antibodies are low down on the list of risks. Just before I retired, there was a move that all RCI Departments would go over to electronic issue, when there was a positive DAT and no detectable underlying atypical alloantibodies, but whether or not this ever went through, I don't know; you would have to contact your local RCI Department. gagpinks 1 Link to comment Share on other sites More sharing options...
Mabel Adams Posted May 30, 2018 Share Posted May 30, 2018 We turn out a negative DTT-treated screen and do electronic crossmatch. If the screen has gone out as positive our computer won't allow EXM so we would do IS as Malcolm suggests. More complicated if there are alloantibodies but we haven't seen that yet. Link to comment Share on other sites More sharing options...
Bb_in_the_rain Posted June 9, 2018 Share Posted June 9, 2018 (edited) On 6/3/2017 at 1:21 AM, Malcolm Needs said: I haven't heard of that method Mabel. What is the "weird" Lutheran antibody. Anti-CD38 did not react with In(Lu) cells and showed very low signals when anti-CD38 was tested with In(Lu) cells in a flow cytometry. Before we knew the patient was on anti-CD38 and In(Lu) cells has depressed CD38 marker, we have mistaken it being an antibody to high incidence antigen in Lutheran blood group system, based on the reactivity with In(Lu) cells. Edited June 9, 2018 by dothandar exlimey and Malcolm Needs 2 Link to comment Share on other sites More sharing options...
SBBSue Posted June 12, 2018 Share Posted June 12, 2018 I've been told that the concentration of DTT in RESt is different that what is used for treatment of reagent cells. But that sure would have been convenient. exlimey 1 Link to comment Share on other sites More sharing options...
exlimey Posted June 13, 2018 Share Posted June 13, 2018 (edited) 20 hours ago, SBBSue said: I've been told that the concentration of DTT in RESt is different that what is used for treatment of reagent cells. But that sure would have been convenient. RESt = Rabbit Erythrocyte Stroma - basically stabilized red cell membranes from rabbits. There is absolute no DTT in RESt. Edited June 13, 2018 by exlimey Typo Malcolm Needs 1 Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now