Jump to content

QC for anti-human globulin, anti- A, Anti-B and Anti-D reagents


mrkeramati

Recommended Posts

If you use FDA licensed reagents (in the USA) - all I would do is verify that they pass my routine QC of such reagents.  There is no need to do parallel testing (at least in my opinion).

we do not use FDA licensed reagents and some time we have encountered with some problems using the reagents. 

Link to comment
Share on other sites

we do not use FDA licensed reagents and some time we have encountered with some problems using the reagents. 

If you are having problems (? lack of reactivity, false positive) I would look at how you are storing the reagents, are they out of the refrigerated environment for long periods of time, are your techs contaminating them somehow, are your serofuges set at the correct time (and have you done the testing to validate the correct spin times for each reagent), is your saline/diluent contaminated?  As you can see there are many variables to consider - HOWEVER, the most important and usually the culprit is the humans.

 

You would normally qc your anti-A, -B, -D reagents with a positive and negative cell.  For your anti-IgG I would use sensitized and non-sensitized rbcs..

 

Don't buy from the low bid if these are the reagents giving you problems.  I always try to purchase the best reagents I can find for blood bank testing.

Edited by David Saikin
Link to comment
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
  • Advertisement

×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.