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Sickle test for patients


Maryam

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Dear Maryam

The way it works is that you have a lyophilised reducing agent that needs to be reconstituted just before use. You mix the cells being tested with the reducing agent and pipette on to a neutral ID card, then centrifuge.  This is not an antibody-antigen reaction, but a physical one.   HbS pos cells are deformed when added to the reducing agent, and are then not flexible enough to travel through the gel as normal cells would be able to do.  It is important to realise it is only a screening test

anna

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