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Comparability of 2 ProVues


Dansket

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CAP Inspection Checklist item TRM.31450 states, "If the laboratory uses more than one instrument/method to test for a given analyte, the instruments/methods are checked against each other at least twice a year for correlation of results".

 

We have two Provues that are used every other day, one ProVue is powered on and the other ProVue is powered off. QC is run daily using commercially available simulated whole blood controls.  The same lot number of control (the same set of four tubes) is used for a 7-day period.  The form used to document this activity reflects that each ProVue is tested with the same lot every other day for two 7-day periods and that QC passed.  Doesn't this show instrument comparability?

 

The inspector stated " Build a spreadsheet that shows the reactivity of each gel card well (anti-A, anti-B, anti-D, D control, etc.) with the individual whole blood controls) for each ProVue instrument for visual comparision on the same sheet of paper.

 

What do you do?

Edited by Dansket
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We take two specimen and run it by two ProVue, manual gel and tube method. (all the tests we run on all platform). This is done twice a year.

I believe you can use QC material but need to document the way inspector stated as your correlation study. Just saying that you are doing it is not acceptable...our world...if it is not documented it is not done!!!

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We take two specimen and run it by two ProVue, manual gel and tube method. (all the tests we run on all platform). This is done twice a year.

I believe you can use QC material but need to document the way inspector stated as your correlation study. Just saying that you are doing it is not acceptable...our world...if it is not documented it is not done!!!

Do you make your comparisions at the tube level, anti-A =3+ anti-B = 4+, etc.  or just at the interpretation level; BPOS, Antibody Screen - Negative?

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I use manual gel and tubes as back up.  I use an ab positive CAP specimen for my comparison so I do 3 a year.  I document reactivity of the cells, whether they are screening cells or ABORH types.  I state in my protocol that I expect pretty equivalent results from the ABORh testing and that with the  antibody testing it is anticipated that gel will display stronger reactions than PeG or Liss.

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  • 1 month later...

 antibody testing it is anticipated that gel will display stronger reactions than PeG or Liss

 

We have discovered after a couple years of these comparison studies at a multi-facility hospital system that PEG is much more sensitive than GEL.  This really surprised us since we expected that they were very similar. 

 

Is your assumption (quoted above) still true for you?

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We have discovered after a couple years of these comparison studies at a multi-facility hospital system that PEG is much more sensitive than GEL.  This really surprised us since we expected that they were very similar. 

 

Is your assumption (quoted above) still true for you?

That has been and is still my experience, though I am certain there are antibodies where PeG will be better. There is no "one" method that is the best.

For even more sensitivity you can try setting up your PeG testing in tubes, washing it a few times and then instead of adding coombs' antisera put it in an IgG card (add ~100uL diluent to your dry button and use 50uL of suspension). Read an article on this in a recent (last 2yrs) TRANSFUSION magazine. (if you want to drive yourself crazy with sensitivity/specificity issues but can be a handy tool on occasion. I am in the process of validating this technique).

Edited by David Saikin
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