First I make a 1:1000 dilution of Anti-D with 22% BSA, I use the earliest exp. date of the BSA or Anti-D as the dilution exp. date. This gives a 2+ in my LISS tube method. Using negative patient plasma I make a 1:10 dilution of the 1:1000. This gives me a good 2+ in gel. I give this a 30 day exp and usually get good stability.