When we have antibodies that we can only ID in one media, it doesnt bother me. (even if it doesnt make sense, like something neg. in Gel but 2+ in LISS) What bothers me, is 3 cells reactive out of 45 in PEG and EVERYTHING else is negative. Enzymes, LISS, Gel, 6 Coombs Xmatch all neg. Thats when I worry. My lack of knowledge and exp. (I have only been blood banker for 10 years) keeps me second guessing myself late into the evening sometimes. I notice many of the workups we receive that are only detectable on homozygous cells in Gel, are on older women (to me that is 75 and older) who have history of multiple pregs. I wonder how clinically significant these are when they get transfused on negative crossmatches. At some point I guess we just have to say, "we have done as much (within reason) as we can to make this transfusion safe " and pray HE will take care of the rest.